Isotype switching

Isotype switching

Isotype switching is often used to alter the effector function of antibodies. By altering the constant regions while keeping the variable regions intact, we can engineer your antibody for specific applications and optimize experiment outcomes.

Examples:
IgG1 -> IgG2 or IgG4
Inhibit ADCC by changing your IgG1 antibody into IgG2 or IgG4. This is typically done to prevent off-target toxicity of a therapeutic antibody.

mIgG1 -> mIgG2a
mIgG2a is the most effective subclass for engaging Fc receptors which makes it a great antibody for study effector function like ADCC or complement-dependent cytotoxicity (CDC) in animal models.